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Environmental conditions (ECs) can be used to define the values of uptake fluxes, i.e. the rates at which metabolites are consumed and thus can be used to define media for the growth of the cells.

Yau can create ECs by accessing the option "File/Create Environmental COnditions".


The interface allows the user to select drain reactions and define their limits. The upper part allows the selection of the reactions (easing its search),while the bottom part allows to add these constraints to the EC, specifying the lower and upper bounds. The list in the bottom part of the interface, keeps the reactions and their limits as they are added to the EC.


The EC is kept in the clipboard under “Project Elements/Environmental Conditions” and it can be used in simulations.