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How to create Environmental Conditions

You can access the "Environmental Conditions" option under the "Simulation" menu.
Simulation menu.png
In the Environmental Conditions you can select the model/project to work, and set up your conditions.
Env conditions.png
In the reactions list you can search the for reactions present in the model, select and change their flux limits (lower bound / upper bound) and consequently add the new constraints to the reaction constraints list. The reactions in this list are typically extra-cellular/ drains fluxes, i.e. they can be used to define the way rates in which external metabolites are consumed or produced.

After you finish you will find a new icon representing the Environmental Conditions in the clipboard.

http://sites.google.com/site/optfluxhowtos/howtos/environmental-conditions/EnvironmentalConditionsSolution.png