To do a Wild-type simulation go to MSeed > Operations > Model > FBA > Wild Type and select the local model-seed to use and the biochemisty and media to be used in the simulation. A simulation result will be created in the OptFlux workspace.
Reaction KO FBA
To perform a reaction knock-out simulation go to MSeed > Operations > Model > FBA > Reaction KO and select:
- the local model-seed
- the biochemisty
- reaction to be turned off in the simulation.
Gene KO FBA