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it either through the Project menu or the Toolbar.<br>
 
it either through the Project menu or the Toolbar.<br>
 
<br>
 
<br>
http://sites.google.com/site/optfluxhowtos/howtos/1/newProjectWizard.png
+
http://sites.google.com/site/optfluxhowtos/howtos/1/newProjectWizard.png</div>
<br><br>
+
<br>
 
<b>1st step</b><br>
 
<b>1st step</b><br>
 
In the 1st step of the wizard you have to enter a name to the project and choose SBML as the model source input.<br>
 
In the 1st step of the wizard you have to enter a name to the project and choose SBML as the model source input.<br>
 +
<b>
 
<div style="display: block; text-align: left;">
 
<div style="display: block; text-align: left;">
http://sites.google.com/site/optfluxhowtos/howtos/1/Step1NewProjectSBML.png</div><br>
+
http://sites.google.com/site/optfluxhowtos/howtos/1/Step1NewProjectSBML.png</div>
<b>2nd step</b><br>
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</b><br>
 
OptFlux
 
OptFlux
allows you to load Standard SBML files and also CellDesigner SBML files.<br>
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allows you to load pure SBML files and also CellDesigner SBML files.<br>
 
In
 
In
 
the 2nd step, you have to define the type of the file, and also choose
 
the 2nd step, you have to define the type of the file, and also choose
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<br>
 
<br>
 
<b>
 
<b>
[[Image:Step2 sbml.png]]
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<div style="display: block; text-align: left;">http://sites.google.com/site/optfluxhowtos/howtos/1/Step2NewProjectSBML.png</div>
</b><br><br>
+
</b><br>
<b>3th step</b><br>
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<b>3rd step</b><br>
To allow OptFlux to determine the external metabolites you can choose to use "Use compartments heuristics" or "Use regular expressions".
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To allow
 +
OptFlux to determine the external metabolites you can choose to use
 +
"Use compartments heuristics" or "Use regular expressions".<br>
 
<br>
 
<br>
The first detects external metabolites based on the definitions of the compartments contained in the SBML file.
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The first detects external metabolites based on the definitions of the compartments contained in the SBML file.<br>
Using the regular expressions option you can easily set up an expression to detect external metabolites from patterns in their names using the options: "starting with" / "ending with" / "contains" / "match regexp".<br>
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Using
You can also load the most commonly used regular expressions using the option "Load common regexps".
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the regular expressions option you can easily set up an expression to
 +
detect external metabolites from patterns in their names using the options: "starting with" /
 +
"ending with" / "contains" / "match regexp".<br>
 +
You can also load the most commonly used regular expressions using the option "Load common regexps".<br>
 
<br>
 
<br>
 +
<div style="display: block; text-align: left;">
 +
<div style="display: block; text-align: left;">http://sites.google.com/site/optfluxhowtos/howtos/1/Step3NewProjectSBML.png</div>
 
<br>
 
<br>
[[Image:Step3 sbml.png]]
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</div>
 
<br>
 
<br>
 +
<b>4th step</b><br>
 +
In the 4th step, you can validate the information regarding external metabolites / drains detected by OptFlux adding or removing elements to the original list.<br>
 
<br>
 
<br>
<b>4th step</b><br>
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<div style="display: block; text-align: left;">http://sites.google.com/site/optfluxhowtos/howtos/1/Step4NewProjectSBML.png</div>
The identification of the reaction corresponding to biomass formation is an essential task for performing simulations. In this step you have to validate the biomass/ growth reaction automatically detected. You can change it if the heuristic used in the detection did not work.<br>
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<br>
 +
<br>
 +
<b>5th step</b><br>
 +
In the 5th step, you have to validate the biomass/ growth flux automaticaly detected. You can change it if the heuristic used in the detection did not work.<br>
 
<br>
 
<br>
[[Image:Step4.png]]
+
<div style="display: block; text-align: left;">http://sites.google.com/site/optfluxhowtos/howtos/1/Step5NewProjectSBML.png</div>
 
<br>
 
<br>
 
<br>
 
<br>

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