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right clicking on the Metabolic Model icon on the clipboard.<br>
 
right clicking on the Metabolic Model icon on the clipboard.<br>
 
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This option allows you to calculate the minimum and maximum possible values for all the fluxes in the model. It also allows to constrain the value of the biomass flux to be larger than a pre-defined value. The simulations are always performed using Flux Balance Analysis (FBA).<br>
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This option allows you to calculate the minimum and maximum possible values for all the fluxes in the model. The simulations are always performed using Flux Balance Analysis (FBA) by maximizing and minimizing each individual flux.<br>
<div style="display: block; text-align: left;">http://sites.google.com/site/optfluxhowtos/howtos/flux-variability-analysis/how-to-calculate-flux-limits/LimitFluxMenu.png</div>
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[[Image:Flux variation menu.png]]
 
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You can select the model/project to work, and set up the calculation options.<br>
 
You can select the model/project to work, and set up the calculation options.<br>
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<b>1.Minimum Biomass</b><br>
 
<b>1.Minimum Biomass</b><br>
Define the minimum value for the biomass. If no contraint is desired, just set this value to zero.<br>
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Define the minimum value for the biomass (in %).<br>
 
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<i><b>2.Select Environmental Conditions</b></i><br>
 
<i><b>2.Select Environmental Conditions</b></i><br>
If you have loaded/created environmental conditions you can select them to be use as additional constraints.<br>
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If you have created environmental conditions you can select them to be use as additional constraints.<br>
 
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Press OK, and check the result in the clipboard.<br>
 
Press OK, and check the result in the clipboard.<br>

Latest revision as of 19:31, 17 November 2009

How to Calculate Flux Limits

You can access the "Calculate Flux Limits" option under the "Simulation -> Flux Variability Analysis" menu or right clicking on the Metabolic Model icon on the clipboard.

This option allows you to calculate the minimum and maximum possible values for all the fluxes in the model. The simulations are always performed using Flux Balance Analysis (FBA) by maximizing and minimizing each individual flux.
Flux variation menu.png
You can select the model/project to work, and set up the calculation options.


1.Minimum Biomass
Define the minimum value for the biomass (in %).

2.Select Environmental Conditions
If you have created environmental conditions you can select them to be use as additional constraints.

Press OK, and check the result in the clipboard.