From Optflux
Warning: You are not logged in. Your IP address will be publicly visible if you make any edits. If you log in or create an account, your edits will be attributed to your username, along with other benefits.
The edit can be undone.
Please check the comparison below to verify that this is what you want to do, and then save the changes below to finish undoing the edit.
Latest revision | Your text | ||
Line 4: | Line 4: | ||
After install the OptFlux, the Reg4OptFlux plugin can be installed through the repository manager present in OptFlux. | After install the OptFlux, the Reg4OptFlux plugin can be installed through the repository manager present in OptFlux. | ||
− | *You can access the '''Repository''' option under the '''Help -> Repository Manager''' menu.<br> | + | *You can access the '''Repository''' option under the '''Help->Repository Manager''' menu.<br> |
[[Image:installregplugin.png|600px]] | [[Image:installregplugin.png|600px]] | ||
− | |||
− | |||
− | |||
=Example file= | =Example file= | ||
− | You can download it here -> [ | + | You can download it here -> [[Media:IntegratedModelToy.zip|IntegratedModelToy.zip]] |
[[Image:IntegratedModel.png]] | [[Image:IntegratedModel.png]] | ||
Line 25: | Line 22: | ||
*At the metabolic level, the substrate (S) is utilized to produce biomass (B) and by-products P1 and P2. The cellular objective is to maximize biomass production (B) and the engineering objective is the production of P1. Reaction R2 converts the internal metabolite I1 into product P1 and 0.08 biomass (B), whereas reaction R5 converts the internal metabolite I2 into product P2 and 0.12 Biomass. The stoichiometric coefficients of all other reactions reflect a one-to-one relationship between molecule quantities. | *At the metabolic level, the substrate (S) is utilized to produce biomass (B) and by-products P1 and P2. The cellular objective is to maximize biomass production (B) and the engineering objective is the production of P1. Reaction R2 converts the internal metabolite I1 into product P1 and 0.08 biomass (B), whereas reaction R5 converts the internal metabolite I2 into product P2 and 0.12 Biomass. The stoichiometric coefficients of all other reactions reflect a one-to-one relationship between molecule quantities. | ||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
= Build a new project containing an integrated model = | = Build a new project containing an integrated model = | ||
Line 76: | Line 31: | ||
[[Image:MakeIntegratedProject.png]] | [[Image:MakeIntegratedProject.png]] | ||
− | + | At the moment it is only possible to build an integrated model using the wizard operation from Flat Files, (new Formats will be available in a near future version). | |
* '''Step 1''' | * '''Step 1''' | ||
− | + | [[Image:Loadprojectfrommodel.png]] | |
− | |||
− | |||
− | |||
* '''Step 2''' | * '''Step 2''' | ||
− | + | [[Image:SelectionModelFiles.png]] | |
− | + | ||
− | |||
− | |||
+ | The user have to choose the the ''toy.fluxes'' file as the '''Reactions File'''; the ''toy.matrix'' file as the '''Matrix File'''; the ''toy.metab'' file as the '''Metabolites File'''; the ''toy.gr'' file as the '''GPR Information''' and the ''toy.regNet'' file as the '''Regulatory Model File'''. | ||
* '''Reader Configuration''' | * '''Reader Configuration''' | ||
− | + | [[Image:ReaderConfiguration.png]] | |
− | |||
− | |||
− | |||
In this step some definitions can be selected (depending from the type of the delimiters that are used on user Flat Files) | In this step some definitions can be selected (depending from the type of the delimiters that are used on user Flat Files) | ||
Line 118: | Line 66: | ||
* The following dialog panel will be displayed | * The following dialog panel will be displayed | ||
− | [[Image: | + | [[Image:NewIntegratedModelProject.png]] |
<b>1. <i>Project</i></b><br> | <b>1. <i>Project</i></b><br> | ||
You can select the model/project to integrate the regulatory model.<br> | You can select the model/project to integrate the regulatory model.<br> | ||
<br> | <br> | ||
− | <b>2. <i> | + | <b>2. <i>Choose Regulatory model reader type</i></b><br> |
− | You can select the regulatory | + | You can select the type of reader for the regulatory network.<br> |
<br> | <br> | ||
− | <b>3. <i> | + | <b>3. <i>Choose Regulatory model file</i></b><br> |
− | You can select | + | You can select the regulatory model file. In our example the regulatory model file name is toy.regNet.<br> |
<br> | <br> | ||
− | |||
After pressing the ''Ok'' button the new project will be created, and the model components will be displayed on '''Clipboard'''. | After pressing the ''Ok'' button the new project will be created, and the model components will be displayed on '''Clipboard'''. |