How to calculate critical genes/reactions

How to calculate a Critical Genes/Reactions

You can access the "Calculate Critical Genes/Reactions" option under the "Model Simplification" menu or doing right click on the Metabolic Model icon on the clipboard.

http://sites.google.com/site/optfluxhowtos/howtos/model-simplification/performing-structural-simplification/CalculateCriticalMenu.png

In the Calculate Critical Genes/Reactions options you can select the model/project to work, and set up your simplification configuration.

http://sites.google.com/site/optfluxhowtos/howtos/model-simplification/performing-structural-simplification/CalculateCriticalOperation.png

1.Biomass Flux
Choose the flux related to the biomass growth.

2.Critical Information
Choose to use reactions or genes (if GRP information available).

3. Select Environmental Conditions
If you have loaded/created environmental conditions you can select them to use as constrains in the critical genes/reactions calculation.

And that's all, now you can press OK and check the results in the clipboard.

http://sites.google.com/site/optfluxhowtos/howtos/model-simplification/performing-structural-simplification/CalculateCriticalSolution.png